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Protein Expression Service

Santascott has an E. coli expression system, a mammalian cell expression system, and a yeast cell expression system to provide customers with abundant recombinant expression resources.


Service cycle


E. coli expression system

The service content

time

deliverables

E.coli.Expression vector construction

1-2weeks

Expression plasmid/construction   report

E.coli. Expression detection

1week

Expression test   report

E.coli.Fermentation, protein purification

3week

Protein/purification report


Mammalian   cell expression system

The service content

time

deliverables

Mammalian cell expression system

1-2weeks

Expression plasmid/construction report

 CHO/HEK293 Instantaneous   transfection and expression detection 

3week

Expression test   report

CHO/HEK293 transient transfection and   protein purification

3week

Protein/purification report

  

Yeast   cell expression system

The service content

time

deliverables

Yeast expression vector construction

1-2weeks

Build report

Yeast expression strain

2week

Positive strains

High copy cell line screening

1 week

High copy strain

Yeast positive cell line expression   assay

3 week

Expression test report

Yeast expression system target   protein expression, purification

3 week

Target protein / purification   report


   Service Process

1.      Select the protein expression system, and the project leader will carry out the needs understanding and program development.

2.      Sign the technical service agreement and collect the project advance payment

3.      Start the project according to the standard service process and regularly report the progress of the project

4.      On-time delivery of project reports and products